B) Maximum intensity projection of an image stack through the leg epithelium marked with E-cadGFP to highlight cell outlines. ouput of the plateness function = 0.6.(TIFF) pcbi.1004124.s003.tiff (322K) GUID:?7D38551D-424E-49F8-A39F-C8F711C18F6C S4 Fig: AJs Vertex location. The output of the Vertexness function here proposed has been overimposed in black over the plateness function outputs shown in S1 Fig at the corresponding scales (A = 0.14, B = 0.45 and C = 0.60). Note that at higher scales (B Amiodarone hydrochloride and C) vertices which are close to each other tend to merge, while at lower scales vertices tend to appear at non-vertex locations along the AJs. A set up such as the one proposed in panel B is desired as it provides an accurate detection of AJs and vertices. In A the scale is too low resulting in high noise, while in C the scale is too high resulting in detection of blurred features.(TIFF) pcbi.1004124.s004.tiff (386K) GUID:?0714812F-9EDB-4B68-8EB4-3FC0A71A489D S5 Fig: Solution to the correspondence among the cells in a hypothetical epithelial tissue. A) Two cells, l2 divides to produces r2 and r3. B) The graph we built to represent all the correspondence hypotheses. Arcs in red represent cell association, in blue cells entering the scene, in green mitosis, in pink apoptosis and in gray cells leaving the scene. C) The arcs of the graph expected to represent the desired solution(TIFF) pcbi.1004124.s005.tiff (279K) Rabbit Polyclonal to CDK5 GUID:?3E2226E2-CDEF-4E4F-B33E-8352BFE43D7C S6 Fig: Common errors of vertex detection. Details from Fig 4C. Green vertices represent true detections, blue, missed detections, and red, false detections. A) Common pattern of vertices detected at bristle locations, where many vertices are not detected but one is falsely detected at the center. B) appear along edges between vertices as regions with high curvature that are detected as vertices.(TIFF) pcbi.1004124.s006.tiff (228K) GUID:?BECAD144-28B2-462C-87E2-74A86AEE668C S7 Fig: Variation of the tracking performance according to the weights given to the distances between the different features. Global shows the harmonic mean of the Average F1-scores obtained for the different datasets. The difference at the optimal between the global measure and the Average F1-scores of each dataset is not significant, but the global measure drops fast as parameter values deviate from Amiodarone hydrochloride the optimal. A) Centroids. B) Area. C) Perimeter. D) Width. E) Rotation. F) Length.(TIFF) pcbi.1004124.s007.tiff (1.2M) GUID:?A8F9C451-869C-4058-A753-14ABF868DC4D S8 Fig: Variation of the tracking performance according to the weights given to the different hypotheses. Similar to S7 Fig, global shows the harmonic mean of the Average F1-scores obtained for the different datasets. The difference at the optimal between the global measure and the Average F1-scores of each dataset is not significant, but the global measure drops fast as parameter values deviate from the optimal. A) Cell Association. B) Cell entering the scene. C) Cell mitosis. D) Cell Apoptosis. E) Cell leaving the scene.(TIFF) pcbi.1004124.s008.tiff (744K) GUID:?23168FA3-3202-4BA0-993C-ADBDCA2A90BC S9 Fig: Values of the optimal weights given to the distance among the different cell features employed to compute cell association hypotheses to track cells. and are respectively the weights given to the the distances among cell centroids, areas, perimeters, widths, rotations and heights to compute cell association costs. The distance between cell centroids (imaginal discs. We demonstrate the utility of the pipeline to extract key quantitative features of cell behavior with which to elucidate the dynamics and biomechanical control of epithelial tissue morphogenesis. We have made our methods and data available as an open-source multiplatform software tool called TTT (http://github.com/morganrcu/TTT) Author Summary Epithelia are the most common tissue type in multicellular organisms. Understanding processes that make them acquire their final shape has implications to pathologies such as cancer progression and birth defects such as spina bifida. During development, epithelial tissues are remodeled by mechanical forces applied at the Adherens Junctions (AJs). The AJs form a belt-like structure below the apical surface that functions to both mechanically link epithelial cells and enable cells to remodel their Amiodarone hydrochloride Amiodarone hydrochloride shape and contacts with.