Following incubation, samples were dialyzed overnight at 4C against two changes of the phosphate buffer (2L each), and then applied to separate aminophenylboronate columns (1
Following incubation, samples were dialyzed overnight at 4C against two changes of the phosphate buffer (2L each), and then applied to separate aminophenylboronate columns (1.5×10 cm) previously equilibrated with 50mM phosphate buffer, Mogroside IVe pH 7.4. Treatment of galactated HSA with sodium borohydride stabilized the condensed sugars on the protein and yielded discrete fragmentation patterns…