CD4+CD25? T cells had been purified using magnetic bead parting and blended with antigen-presenting cells depleted of Compact disc4+ cells retrieved in the spleens of FTY720- or vehicle-treated mice
CD4+CD25? T cells had been purified using magnetic bead parting and blended with antigen-presenting cells depleted of Compact disc4+ cells retrieved in the spleens of FTY720- or vehicle-treated mice. continuing, these T cells became unresponsive to restimulation with antigen in vitro, which anergic condition was reversed by addition of interleukin 2. Measurements of Compact disc4+Compact…