Cells ingredients were resolved by SDS-PAGE then, and receptors had been visualized by Traditional western blotting using an anti-FLAG antibody
Cells ingredients were resolved by SDS-PAGE then, and receptors had been visualized by Traditional western blotting using an anti-FLAG antibody. Arrowheads indicate the glycosylated mature type of V2R fully. remedies. Blocking endocytosis exacerbated the raised AB-680 basal cAMP amounts marketed by R137C/L-V2R however, not the cAMP creation elicited by R137H-V2R, demonstrating that substitution of Arg137…