pylori infection and upper respiratory diseases (11C14)

pylori infection and upper respiratory diseases (11C14). A possible role of H. IgG&IgA (Fisher’s CNT2 inhibitor-1 Exact test=0.3; 0.5). Conclusion Positive PCR in adenoid tissue (15%) was very close to positive serum IgA (15%) but without any agreement for each case. The H. pylori infection may have a relative role at least in 15% of children with adenoid surgery. Chronic sinusitis and ear infection might be added to infected adenoid tissue as a reservoir for bacteria. The search by specific culture may elucidate better the role of H. pylori infections in both gastric and adenoid tissues. CNT2 inhibitor-1 The decision for use of antibiotics to eradicate the H. pylori infection in recurrent or chronic adenotonsillar infections (with rhinosinusitis) before adenoid surgery needs Randomized Control Trial (RCT) studies. Drug of choice for eradication of H. pylori dependent to antibiotic sensitivity test in each country. Mouse monoclonal to PROZ reported the prevalence of H. pylori Infection among Children in Rasht (north of Iran) (20). Some studies reported the association between H. pylori infection and upper respiratory diseases (e.g. chronic rhinosinusitis; chronic otitis media; chronic otitis media with effusion) (11C14) but little is known about the true colonization and the localization of these bacteria in the adenoid tissue of children in Iran. The study goal was searching the H. pylori infection in adenoid tissue and serum IgA, IgG antibodies in children with adenoid surgery. Methods A cross- sectional study had done on 53 children with CNT2 inhibitor-1 adenoid surgery in ENT and Pediatric Department of Rasul Akram Hospital during 2008-2010. This study was approved by the Ethical Committee in the ENT and head &Neck Research Center in Tehran University of Medical Sciences. (Ethical Considerations detail in the end of article). Initially, a questionnaire was completed by an authorized physician, followed by complete clinical examinations. Before surgery, all cases were visited by a pediatric specialist to check for other concomitant disorders (immunodeficiencies, diabetes mellitus, renal/heart failure, etc.). We excluded all cases with proven immunodeficiency, diabetes mellitus, renal failure, patients who had received antibiotics up to 2 weeks before surgery, and cases with known malignancy or other diseases proven in pathological studies. 2 ml Blood samples were centrifuged and the serum stored in a freezer at C20C for the serological examination. The study group consisted of 53 children (Mean age=8 1.9 years) with recurrent or chronic adenotonsillar infections candidate for adenoid surgery selected continuously. Of 53 cases with adenoid surgery, 40 cases had rhino sinusitis (in sinus CT scan). Specific H. pylori antibodies (IgG and IgA) were investigated by ELISA assay in all cases and controls. Using commercial kits (Chemicon-Germany), the results were interpreted quantitatively as recommended by the manufacturer. During surgery, 1 cm3 of adenoid tissue resected and put in sterile tubes and then they were centrifuged, homogenized in the tubes and stored in a freezer at C80C. The polymerase chain reaction (PCR) template purification kit (Roche Diagnostics GmbH, Germany) was used for all prepared tissue samples. The contents of the binding column tube were transferred to a new sterile 1.5 ml tube, after which the integrity of the DNA assessed by gel electrophoresis (1% agarose gel). H. pylori DNAs were identified by qualitative specific PCR primer kits (QIA quickP? QIAGEN; Germany). Diagnostic kits included ready-to-use PCR mix kits, positive and negative controls and other qualified reagents along with an easy to follow protocol to detect the H. pylori genome at as low as10 copies. H. pylori: primers 93089 and 93261 were selected from consensus regions of the two available cag A gene sequences (GenBank accession no. “type”:”entrez-nucleotide”,”attrs”:”text”:”L11714″,”term_id”:”290950″,”term_text”:”L11714″L11714 and EMBL accession no.}L11714.} 70039) 400 bp product Cag A gene: Forward: AAT ACA CCA ACG CCT CCA AG Reverse: TTG TTG CCG CTT TTG CTC TC Statistical analysis The student’s t-test was used to determine significant differences in means for continuous variables and the Chi-squared test was used to compare categorical data CNT2 inhibitor-1 in cases and controls. P values less than 0.{05 were considered to be statistically significant.|05 were considered to be significant statistically.} {The agreement between the serologic test and PCR was assessed by.|The agreement between the serologic PCR and test was assessed by.}